Information
Accession GMS-64-74 (FID: 661)Browse (ALL)
Title Hi-C on WTC-11 cardiac differention timecourse
HiC file 4DNFI8QUAO51 (6.55 GB)
 hic  mCool   Cooler-Tree
Submit date2025-02-18 15:23:10
Last update date2025-02-18 15:23:10
Contact Sung-Joon PARK
park AT hgc.jp
The University of Tokyo
Sharingopen access
Keywords Hi-C  4DN  contact matrix 
 Domain/Loop Calling Information

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Submit date 2025-02-18 15:23:10
Reference hg38 (Human)  
Result file DomainCall.tar.gz (354.58 MB)  
Insulation Score (4DN) 10000bin_100000win_chrAdded.bw
5000bin_100000win_chrAdded.bw
GMS Domain 7765 domains
13675 boundaries
GMS Loop 17146 loops

Contact Matrix
Large JuiceBox 
JuiceBox-web 
Motifs in 5Kb Boundaries (13675)
CTCF(+) RAD21(-)  529 (3.87%)
CTCF(-) RAD21(+)  378 (2.76%)
CTCF(+) RAD21(+)  1865 (13.64%)
GimmeMotifs 
(Pvalue < 0.001, Top10)
JASPAR#. hits-log10
Pvalue		ROC
AUC
CTCF 486 39.044 0.543
ZBTB2 344 17.436 0.537
Sox5 719 11.066 0.544
HOXB6 463 11.047 0.517
NKX6-1 462 10.487 0.510
HOXB1 427 10.372 0.511
FOXD1 506 9.932 0.516
FOXL1 471 9.759 0.523
FOXI1 456 9.378 0.523
FOXO4 456 9.378 0.521
 CTCF&RAD21 in Boundaries
 Raw ROC file
 Enriched_motifs.html
 All_motifs.html
Motifs in 5Kb Loop Anchors (17146 x2 anchors)
CTCF(+) RAD21(-)  772 (4.5%) LA
 780 (4.55%) RA
 33 (0.19%) LA & RA
CTCF(-) RAD21(+)  531 (3.1%) LA
 504 (2.94%) RA
 18 (0.1%) LA & RA
CTCF(+) RAD21(+)  4572 (26.67%) LA
 4480 (26.13%) RA
 1591 (9.28%) LA + RA
CTCF directionality
(17167 cases)		  1498 (8.73%)Convergent ≫ ≪
 65 (0.38%)Divergent ≪ ≫
 557 (3.24%)Tandem ≫ ≫
 6406 (37.32%)Singleton ≫
GimmeMotifs 
(Pvalue < 0.001, Top10) LA+RA
JASPAR#. hits-log10
Pvalue		ROC
AUC
CTCF2605177.3220.624
ZBTB22115128.6990.610
Sox5247043.3110.560
ZNF354C306239.0240.496
FOXL1160231.0470.522
FOXO6153228.1420.523
FOXI1153128.0850.525
FOXO4153128.0850.523
FOXP3153128.0850.520
Nr2e3141024.0170.506
 CTCF&RAD21 in Loop Anchors
 CTCF directionality
 Raw ROC file
 Enriched_motifs.html
 All_motifs.html
 Figures
APA (5K)
 normedAPA (5K)
 centerNormedAPA (5K)
 rankAPA (5K)
APA (10K)
 normedAPA (10K)
 centerNormedAPA (10K)
 rankAPA (10K)
APA (25K)
 normedAPA (25K)
 centerNormedAPA (25K)
 rankAPA (25K)
%>4DN_get_insulation_scores_and_boundaries --bweak 0.2 --bstrong 0.5 --cutoff 2 --pixels_frac 0.66
  "--binsize 5000 --window 100000,--binsize 10000 --window 100000"

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar arrowhead --ignore-sparsity --threads 4 -k KR -m 2000

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar hiccups --ignore-sparsity --cpu --threads 4 -k KR -m 1024 -f .1,.1,.1 -p 4,2,1 -i 7,5,3 -t 0.02,1.5,1.75 -d 20000,20000,50000 -r 5000,10000,25000

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar apa --ignore-sparsity --threads 4

%>hicDetectLoops --threads 2 --peakWidth 2 --windowSize 5 --pValuePreselection 0.1 --peakInteractionsThreshold 10 --obsExpThreshold 1.5 --pValue 0.025 --maxLoopDistance 2000000 --threadsPerChromosome 1 --expected mean

%>cooltools insulation --ignore-diags 2 --append-raw-scores --min-frac-valid-pixels 0.66 --min-dist-bad-bin 0 --chunksize 2000000
  win= 100000

Creating GMS_Domains.bedpe: "hicMergeDomains" with all Strong boundaries of 4DN and "intersectBed" -a all_ArrowHeads -b Strong_boundaries
%>hicMergeDomains --value 5000

Creating GMS_Loops.bedgraph: "hicMergeLoops" of hicCups merged_loops + all_hicDetectLoops -r 25000
%>hicMergeLoops -r 25000

CTCF&RAD21
%>gimme scan -gc -f 0.1 -b -N 1
Boundaries
%>gimme motifs -b genomic -s 0 -f 0.2 -t Homer --known -p JASPAR2020_vertebrates -N 1
Loops
%>gimme motifs -b genomic -s 5000 -f 0.2 -t Homer --known -p JASPAR2020_vertebrates -N 1