Information
Accession GMS-65-15 (FID: 461)Browse (ALL)
Title Liquified Chromatin Hi-C on K562 cells
HiC file 4DNFIDBIEXI7 (2.15 GB)
 hic  mCool   Cooler-Tree
Submit date2025-01-16 11:29:21
Last update date2025-01-16 11:29:21
Contact GMSuite GENOMEMODALITY
gmsuite AT hgc.jp
The University of Tokyo
Sharingopen access
Keywords Hi-C  4DN  contact matrix 
 Domain/Loop Calling Information

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Submit date 2025-01-16 11:29:21
Reference hg38 (Human)  
Result file DomainCall.tar.gz (229.89 MB)  
Insulation Score (4DN) 10000bin_100000win_chrAdded.bw
5000bin_100000win_chrAdded.bw
GMS Domain 2969 domains
5536 boundaries
GMS Loop 6763 loops
 Hi-C map is too sparse

Contact Matrix
Large JuiceBox 
JuiceBox-web 
Motifs in 5Kb Boundaries (5536)
CTCF(+) RAD21(-)  142 (2.57%)
CTCF(-) RAD21(+)  169 (3.05%)
CTCF(+) RAD21(+)  621 (11.22%)
GimmeMotifs 
(Pvalue < 0.001, Top10)
JASPAR#. hits-log10
Pvalue		ROC
AUC
CTCF 154 15.549 0.537
ZBTB2 145 13.455 0.536
Hmx1 93 3.675 0.515
FOXN3 171 3.057 0.524
 CTCF&RAD21 in Boundaries
 Raw ROC file
 Enriched_motifs.html
 All_motifs.html
Motifs in 5Kb Loop Anchors (6763 x2 anchors)
CTCF(+) RAD21(-)  263 (3.89%) LA
 253 (3.74%) RA
 13 (0.19%) LA & RA
CTCF(-) RAD21(+)  224 (3.31%) LA
 226 (3.34%) RA
 11 (0.16%) LA & RA
CTCF(+) RAD21(+)  1007 (14.89%) LA
 1071 (15.84%) RA
 214 (3.16%) LA + RA
CTCF directionality
(6763 cases)		  180 (2.66%)Convergent ≫ ≪
 24 (0.35%)Divergent ≪ ≫
 116 (1.72%)Tandem ≫ ≫
 1954 (28.89%)Singleton ≫
GimmeMotifs 
(Pvalue < 0.001, Top10) LA+RA
JASPAR#. hits-log10
Pvalue		ROC
AUC
CTCF48038.7900.554
ZBTB243932.2370.545
Nr2e357816.3000.508
FOXI153313.5180.531
FOXO653313.5180.530
FOXO453213.4250.531
FOXP353213.4250.528
FOXL154713.3400.526
MEIS158712.4030.528
HOXB652711.4030.506
 CTCF&RAD21 in Loop Anchors
 CTCF directionality
 Raw ROC file
 Enriched_motifs.html
 All_motifs.html
 Figures
APA (5K)
 normedAPA (5K)
 centerNormedAPA (5K)
 rankAPA (5K)
APA (10K)
 normedAPA (10K)
 centerNormedAPA (10K)
 rankAPA (10K)
APA (25K)
 normedAPA (25K)
 centerNormedAPA (25K)
 rankAPA (25K)
%>4DN_get_insulation_scores_and_boundaries --bweak 0.2 --bstrong 0.5 --cutoff 2 --pixels_frac 0.66
  "--binsize 5000 --window 100000,--binsize 10000 --window 100000"

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar arrowhead --ignore-sparsity --threads 4 -k KR -m 2000

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar hiccups --ignore-sparsity --cpu --threads 4 -k KR -m 1024 -f .1,.1,.1 -p 4,2,1 -i 7,5,3 -t 0.02,1.5,1.75 -d 20000,20000,50000 -r 5000,10000,25000

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar apa --ignore-sparsity --threads 4

%>hicDetectLoops --threads 2 --peakWidth 2 --windowSize 5 --pValuePreselection 0.1 --peakInteractionsThreshold 10 --obsExpThreshold 1.5 --pValue 0.025 --maxLoopDistance 2000000 --threadsPerChromosome 1 --expected mean

%>cooltools insulation --ignore-diags 2 --append-raw-scores --min-frac-valid-pixels 0.66 --min-dist-bad-bin 0 --chunksize 2000000
  win= 100000

Creating GMS_Domains.bedpe: "hicMergeDomains" with all Strong boundaries of 4DN and "intersectBed" -a all_ArrowHeads -b Strong_boundaries
%>hicMergeDomains --value 5000

Creating GMS_Loops.bedgraph: "hicMergeLoops" of hicCups merged_loops + all_hicDetectLoops -r 25000
%>hicMergeLoops -r 25000

CTCF&RAD21
%>gimme scan -gc -f 0.1 -b -N 1
Boundaries
%>gimme motifs -b genomic -s 0 -f 0.2 -t Homer --known -p JASPAR2020_vertebrates -N 1
Loops
%>gimme motifs -b genomic -s 5000 -f 0.2 -t Homer --known -p JASPAR2020_vertebrates -N 1