Information
Accession GMS-65-76 (FID: 528)Browse (ALL)
Title Hi-C on sync. HeLa cells - Rep3
HiC file 4DNFI2RN3WFP (1.19 GB)
 hic  mCool   Cooler-Tree
Submit date2025-01-18 01:12:31
Last update date2025-01-18 01:12:31
Contact GMSuite GENOMEMODALITY
gmsuite AT hgc.jp
The University of Tokyo
Sharingopen access
Keywords Hi-C  4DN  contact matrix 
 Domain/Loop Calling Information

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Submit date 2025-01-18 01:12:32
Reference hg38 (Human)  
Result file DomainCall.tar.gz (191.73 MB)  
Insulation Score (4DN) 10000bin_100000win_chrAdded.bw
5000bin_100000win_chrAdded.bw
GMS Domain 498 domains
981 boundaries
GMS Loop 1556 loops
 Hi-C map is too sparse

Contact Matrix
Large JuiceBox 
JuiceBox-web 
Motifs in 5Kb Boundaries (981)
CTCF(+) RAD21(-)  24 (2.45%)
CTCF(-) RAD21(+)  34 (3.47%)
CTCF(+) RAD21(+)  101 (10.3%)
GimmeMotifs 
(Pvalue < 0.001, Top10)
JASPAR#. hits-log10
Pvalue		ROC
AUC
CTCF 24 3.656 0.542
ZBTB2 23 3.304 0.542
 CTCF&RAD21 in Boundaries
 Raw ROC file
 Enriched_motifs.html
 All_motifs.html
Motifs in 5Kb Loop Anchors (1556 x2 anchors)
CTCF(+) RAD21(-)  56 (3.6%) LA
 66 (4.24%) RA
 4 (0.26%) LA & RA
CTCF(-) RAD21(+)  49 (3.15%) LA
 45 (2.89%) RA
 1 (0.06%) LA & RA
CTCF(+) RAD21(+)  204 (13.11%) LA
 223 (14.33%) RA
 26 (1.67%) LA + RA
CTCF directionality
(1556 cases)		  22 (1.41%)Convergent ≫ ≪
 7 (0.45%)Divergent ≪ ≫
 23 (1.48%)Tandem ≫ ≫
 445 (28.6%)Singleton ≫
GimmeMotifs 
(Pvalue < 0.001, Top10) LA+RA
JASPAR#. hits-log10
Pvalue		ROC
AUC
MEIS132621.8110.519
ZNF354C20221.5750.498
Nr2e318321.3910.526
FOXL117920.6580.521
FOXI116718.7690.530
FOXO416718.7690.529
FOXO616718.7690.531
FOXP316718.7690.525
NFATC211516.8560.578
NKX6-116715.4580.500
 CTCF&RAD21 in Loop Anchors
 CTCF directionality
 Raw ROC file
 Enriched_motifs.html
 All_motifs.html
 Figures
%>4DN_get_insulation_scores_and_boundaries --bweak 0.2 --bstrong 0.5 --cutoff 2 --pixels_frac 0.66
  "--binsize 5000 --window 100000,--binsize 10000 --window 100000"

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar arrowhead --ignore-sparsity --threads 4 -k KR -m 2000

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar hiccups --ignore-sparsity --cpu --threads 4 -k KR -m 1024 -f .1,.1,.1 -p 4,2,1 -i 7,5,3 -t 0.02,1.5,1.75 -d 20000,20000,50000 -r 5000,10000,25000

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar apa --ignore-sparsity --threads 4

%>hicDetectLoops --threads 2 --peakWidth 2 --windowSize 5 --pValuePreselection 0.1 --peakInteractionsThreshold 10 --obsExpThreshold 1.5 --pValue 0.025 --maxLoopDistance 2000000 --threadsPerChromosome 1 --expected mean

%>cooltools insulation --ignore-diags 2 --append-raw-scores --min-frac-valid-pixels 0.66 --min-dist-bad-bin 0 --chunksize 2000000
  win= 100000

Creating GMS_Domains.bedpe: "hicMergeDomains" with all Strong boundaries of 4DN and "intersectBed" -a all_ArrowHeads -b Strong_boundaries
%>hicMergeDomains --value 5000

Creating GMS_Loops.bedgraph: "hicMergeLoops" of hicCups merged_loops + all_hicDetectLoops -r 25000
%>hicMergeLoops -r 25000

CTCF&RAD21
%>gimme scan -gc -f 0.1 -b -N 1
Boundaries
%>gimme motifs -b genomic -s 0 -f 0.2 -t Homer --known -p JASPAR2020_vertebrates -N 1
Loops
%>gimme motifs -b genomic -s 5000 -f 0.2 -t Homer --known -p JASPAR2020_vertebrates -N 1