Information
Accession GMS-65-99 (FID: 551)Browse (ALL)
Title Hi-C on HeLa cells - protocol variations
HiC file 4DNFIM2BMJ33 (1.91 GB)
 hic  mCool   Cooler-Tree
Submit date2025-01-18 09:36:07
Last update date2025-01-18 09:36:07
Contact GMSuite GENOMEMODALITY
gmsuite AT hgc.jp
The University of Tokyo
Sharingopen access
Keywords Hi-C  4DN  contact matrix 
 Domain/Loop Calling Information

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Submit date 2025-01-18 09:36:07
Reference hg38 (Human)  
Result file DomainCall.tar.gz (223.26 MB)  
Insulation Score (4DN) 10000bin_100000win_chrAdded.bw
5000bin_100000win_chrAdded.bw
GMS Domain 3406 domains
6323 boundaries
GMS Loop 5795 loops
 Hi-C map is too sparse

Contact Matrix
Large JuiceBox 
JuiceBox-web 
Motifs in 5Kb Boundaries (6323)
CTCF(+) RAD21(-)  186 (2.94%)
CTCF(-) RAD21(+)  199 (3.15%)
CTCF(+) RAD21(+)  759 (12%)
GimmeMotifs 
(Pvalue < 0.001, Top10)
JASPAR#. hits-log10
Pvalue		ROC
AUC
CTCF 186 18.949 0.538
ZBTB2 172 15.789 0.539
ZNF454 104 3.628 0.539
ZNF534 100 3.133 0.511
 CTCF&RAD21 in Boundaries
 Raw ROC file
 Enriched_motifs.html
 All_motifs.html
Motifs in 5Kb Loop Anchors (5795 x2 anchors)
CTCF(+) RAD21(-)  171 (2.95%) LA
 172 (2.97%) RA
 10 (0.17%) LA & RA
CTCF(-) RAD21(+)  175 (3.02%) LA
 190 (3.28%) RA
 8 (0.14%) LA & RA
CTCF(+) RAD21(+)  804 (13.87%) LA
 857 (14.79%) RA
 159 (2.74%) LA + RA
CTCF directionality
(5795 cases)		  127 (2.19%)Convergent ≫ ≪
 18 (0.31%)Divergent ≪ ≫
 81 (1.4%)Tandem ≫ ≫
 1552 (26.78%)Singleton ≫
GimmeMotifs 
(Pvalue < 0.001, Top10) LA+RA
JASPAR#. hits-log10
Pvalue		ROC
AUC
CTCF38632.2710.542
ZBTB231019.7300.540
NKX6-150316.2430.505
Sox574416.1150.554
LHX941315.1820.502
PRRX241514.8540.503
NKX6-239914.2930.502
PRRX142914.1050.504
LBX139413.8990.502
Nr2e346913.2490.500
 CTCF&RAD21 in Loop Anchors
 CTCF directionality
 Raw ROC file
 Enriched_motifs.html
 All_motifs.html
 Figures
APA (5K)
 normedAPA (5K)
 centerNormedAPA (5K)
 rankAPA (5K)
APA (10K)
 normedAPA (10K)
 centerNormedAPA (10K)
 rankAPA (10K)
APA (25K)
 normedAPA (25K)
 centerNormedAPA (25K)
 rankAPA (25K)
%>4DN_get_insulation_scores_and_boundaries --bweak 0.2 --bstrong 0.5 --cutoff 2 --pixels_frac 0.66
  "--binsize 5000 --window 100000,--binsize 10000 --window 100000"

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar arrowhead --ignore-sparsity --threads 4 -k KR -m 2000

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar hiccups --ignore-sparsity --cpu --threads 4 -k KR -m 1024 -f .1,.1,.1 -p 4,2,1 -i 7,5,3 -t 0.02,1.5,1.75 -d 20000,20000,50000 -r 5000,10000,25000

%>java -Xmx32g -jar /usr/proj/gmsuite/TOOL/Juicer/scripts/common/juicer_tools.jar apa --ignore-sparsity --threads 4

%>hicDetectLoops --threads 2 --peakWidth 2 --windowSize 5 --pValuePreselection 0.1 --peakInteractionsThreshold 10 --obsExpThreshold 1.5 --pValue 0.025 --maxLoopDistance 2000000 --threadsPerChromosome 1 --expected mean

%>cooltools insulation --ignore-diags 2 --append-raw-scores --min-frac-valid-pixels 0.66 --min-dist-bad-bin 0 --chunksize 2000000
  win= 100000

Creating GMS_Domains.bedpe: "hicMergeDomains" with all Strong boundaries of 4DN and "intersectBed" -a all_ArrowHeads -b Strong_boundaries
%>hicMergeDomains --value 5000

Creating GMS_Loops.bedgraph: "hicMergeLoops" of hicCups merged_loops + all_hicDetectLoops -r 25000
%>hicMergeLoops -r 25000

CTCF&RAD21
%>gimme scan -gc -f 0.1 -b -N 1
Boundaries
%>gimme motifs -b genomic -s 0 -f 0.2 -t Homer --known -p JASPAR2020_vertebrates -N 1
Loops
%>gimme motifs -b genomic -s 5000 -f 0.2 -t Homer --known -p JASPAR2020_vertebrates -N 1