Information
Accession GMS-65-3 

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TitleDegradation of RAD21 in HCT-116
Submit date2023-05-29 15:56:40
Last update date2023-05-29 15:54:56
Contact GMSuite GENOMEMODALITY
gmsuite AT hgc.jp
The University of Tokyo
Sharing
Total file size1.88 GB
Keywords Cohesin  ChIP-seq  Micro-C  EU-seq  RAD21 
 Study
Experiment type
An auxin-inducible degron system destroys RAD21 in HCT-116 cell line.
RAD21 is a core component of the cohesin complex.
HCT-116 is a human colorectal carcinoma cell line.
Auxin (IAA) treated samples.
Summary After Auxin (IAA) Treatment
ChIP-seq
AFF4, BRD4, CBP, CDK9, MAU2, MED1, NCBP1, NELFCD, RPB1, RAD21, S2P, SPT4, TAF1, TBP, P300, H3K27ac, H3K4me1

EU-seq 
in vivo 5-ethynyluridine (EU) labeling of nascent transcripts

Micro-C

Citation(s)
 Experiment
OrganismHuman
Cell (Tissue) HCT-116
Protocol
Data processing
 Analysis
[1] EU-seq (IAA treated, Rep1)
Browse  Browse (All)
Human (hg38); RNA-Seq

BAM

accepted_hits.bam.sorted.bam (1.81 GB)  
[2] AFF4 narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

AFF4_np1_peaks.narrowPeak.scaled.bigwig (530.03 KB)  
[3] BRD4 narrow peaks in HCT-116 (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

BRD4_np1_peaks.narrowPeak.scaled.bigwig (3.35 MB)  
[4] CBP narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

CBP_np1_peaks.narrowPeak.scaled.bigwig (2.46 MB)  
[5] CDK9 narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

CDK9_np1_peaks.narrowPeak.scaled.bigwig (1.82 MB)  
[6] H3K27ac narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

H3K27ac_np1_peaks.narrowPeak.scaled.bigwig (6.00 MB)  
[7] H3K4me1 narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

H3K4me1_np1_peaks.narrowPeak.scaled.bigwig (8.00 MB)  
[8] MAU2 narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

MAU2_np1_peaks.narrowPeak.scaled.bigwig (395.77 KB)  
[9] MED1 narrow peaks (IAA treated, rep1)
Browse
Human (hg38); ChIP-Seq

BW

MED1_np1_peaks.narrowPeak.scaled.bigwig (3.38 MB)  
[10] MED1 narrow peaks (IAA treated, rep2)
Browse
Human (hg38); ChIP-Seq

BW

MED1_np2_peaks.narrowPeak.scaled.bigwig (2.23 MB)  
[11] NCBP1 narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

NCBP1_np1_peaks.narrowPeak.scaled.bigwig (1.63 MB)  
[12] NELFCD narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

NELFCD_np1_peaks.narrowPeak.scaled.bigwig (2.16 MB)  
[13] P300 narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

P300_np1_peaks.narrowPeak.scaled.bigwig (1.87 MB)  
[14] RPB1 narrow peaks (IAA treated, rep1)
Browse
Human (hg38); ChIP-Seq

BW

RPB1_np1_peaks.narrowPeak.scaled.bigwig (11.92 MB)  
[15] RPB1 narrow peaks (IAA treated, rep2)
Browse
Human (hg38); ChIP-Seq

BW

RPB1_np2_peaks.narrowPeak.scaled.bigwig (7.01 MB)  
[16] S2P narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

S2P_np1_peaks.narrowPeak.scaled.bigwig (9.17 MB)  
[17] SPT4 narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

SPT4_np1_peaks.narrowPeak.scaled.bigwig (7.25 MB)  
[18] TAF1 narrow peaks (IAA treated)
Browse
Human (hg38); ChIP-Seq

BW

TAF1_np1_peaks.narrowPeak.scaled.bigwig (2.75 MB)  
[19] TBP narrow peaks (IAA treated, rep1)
Browse
Human (hg38); ChIP-Seq

BW

TBP_np1_peaks.narrowPeak.scaled.bigwig (3.13 MB)  
[20] TBP narrow peaks (IAA treated, rep2)
Browse
Human (hg38); ChIP-Seq

BW

TBP_np2_peaks.narrowPeak.scaled.bigwig (3.04 MB)  
 Supplements
[390] accepted_hits.bam.sorted.bam
 EU-seq (IAA treated, Rep1)
 application/octet-stream 1.81 GB MD5: f2a8e2cc80d5190370646f88d34aabf5
[356] AFF4_np1_peaks.narrowPeak.scaled.bigwig
 AFF4 narrow peaks (IAA treated)
 application/octet-stream 530.03 KB MD5: 3a15813fe609551fa9375c3af28e1b4b
[359] BRD4_np1_peaks.narrowPeak.scaled.bigwig
 BRD4 narrow peaks in HCT-116 (IAA treated)
 application/octet-stream 3.35 MB MD5: ed6f68db234d9a9f03410138e16af102
[362] CBP_np1_peaks.narrowPeak.scaled.bigwig
 CBP narrow peaks (IAA treated)
 application/octet-stream 2.46 MB MD5: f6886a970a3829b0a54aa3f2be58b638
[364] CDK9_np1_peaks.narrowPeak.scaled.bigwig
 CDK9 narrow peaks (IAA treated)
 application/octet-stream 1.82 MB MD5: c953798eff2948909ad63de4d0f1c1c1
[387] H3K27ac_np1_peaks.narrowPeak.scaled.bigwig
 H3K27ac narrow peaks (IAA treated)
 application/octet-stream 6.00 MB MD5: 99e14d3c29ffabf5060cb2ab3ad0ddbf
[388] H3K4me1_np1_peaks.narrowPeak.scaled.bigwig
 H3K4me1 narrow peaks (IAA treated)
 application/octet-stream 8.00 MB MD5: 206b58313e4b6e786faba73e5d98f786
[363] MAU2_np1_peaks.narrowPeak.scaled.bigwig
 MAU2 narrow peaks (IAA treated)
 application/octet-stream 395.77 KB MD5: 49881dedd458e926a92b443c44bfc171
[366] MED1_np1_peaks.narrowPeak.scaled.bigwig
 MED1 narrow peaks (IAA treated, rep1)
 application/octet-stream 3.38 MB MD5: e301384f20ad868b1c92d6ea11000288
[365] MED1_np2_peaks.narrowPeak.scaled.bigwig
 MED1 narrow peaks (IAA treated, rep2)
 application/octet-stream 2.23 MB MD5: c5333373b109b90a439c99cc71fc0170
[375] NCBP1_np1_peaks.narrowPeak.scaled.bigwig
 NCBP1 narrow peaks (IAA treated)
 application/octet-stream 1.63 MB MD5: 87d3214c010102b29ddbb919b3329964
[377] NELFCD_np1_peaks.narrowPeak.scaled.bigwig
 NELFCD narrow peaks (IAA treated)
 application/octet-stream 2.16 MB MD5: 2485640bb917c35a333923ae5918b0fa
[376] P300_np1_peaks.narrowPeak.scaled.bigwig
 P300 narrow peaks (IAA treated)
 application/octet-stream 1.87 MB MD5: 1abed88db85d2e4e5ffc9e8a37047ae3
[383] RPB1_np1_peaks.narrowPeak.scaled.bigwig
 RPB1 narrow peaks (IAA treated, rep1)
 application/octet-stream 11.92 MB MD5: f55d40c96229979003c13d943cdd4700
[381] RPB1_np2_peaks.narrowPeak.scaled.bigwig
 RPB1 narrow peaks (IAA treated, rep2)
 application/octet-stream 7.01 MB MD5: b95ca6cbe16dd34d8c1e84e0046a9dd1
[382] S2P_np1_peaks.narrowPeak.scaled.bigwig
 S2P narrow peaks (IAA treated)
 application/octet-stream 9.17 MB MD5: 2004a9f54c271d81832b194ac815981f
[384] SPT4_np1_peaks.narrowPeak.scaled.bigwig
 SPT4 narrow peaks (IAA treated)
 application/octet-stream 7.25 MB MD5: 3718fbedf9095dc625ebabbea1ec5474
[378] TAF1_np1_peaks.narrowPeak.scaled.bigwig
 TAF1 narrow peaks (IAA treated)
 application/octet-stream 2.75 MB MD5: 72e870b15ff4f642c79655bb93dcebb4
[379] TBP_np1_peaks.narrowPeak.scaled.bigwig
 TBP narrow peaks (IAA treated, rep1)
 application/octet-stream 3.13 MB MD5: b198517faf80cd1d42548abdf2107dd3
[380] TBP_np2_peaks.narrowPeak.scaled.bigwig
 TBP narrow peaks (IAA treated, rep2)
 application/octet-stream 3.04 MB MD5: d79b35831fc7638902a85fb1c7d11f7f