| Accession | GMS-82-2
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| Title | DNA droplet phase separation with environmental miRNAs for biosensing |
| Submit date | 2025-05-14 02:15:48 |
| Last update date | 2025-05-14 14:45:04 |
| Contact | Masahiro TAKINOUE masahiro.takinoue AT takinoue-lab.jp Institute of Science Tokyo |
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| Total file size | 267.41 KB |
| Keywords | Synthetic DNA Nanoscale DNA droplet DNA DNA nanotechnology |
| Experiment type | In vitro experiments for the formation of artificial liquid-liquid phase separation droplets of DNAs and application to biosensing |
| Summary | This study reports a computational DNA droplet that can recognize a specific combination of tumor biomarker microRNAs (miRNAs) as molecular inputs and output a DNA logic computing result by physical DNA droplet phase separation. A mixed DNA droplet consisting of three DNA nanostructures with orthogonal sticky-end sequences and two linker DNAs to cross-bridge the orthogonal DNA nanostructures is proposed. By the hybridization of miRNAs with the linkers, the cross-bridging ability is lost, causing the phase-separation of the mixed DNA droplet into three DNA droplets, resulting in executing a miRNA pattern recognition described by a logical expression ((miRNA-1 ∧ miRNA-2) ∧ (miRNA-3 ∧ ¬miRNA-4)). This experimentally demonstrates that the computational DNA droplets recognize the above specific pattern of chemically synthesized miRNA sequences as a model experiment. |
| Citation(s) | Jing Gong, Nozomi Tsumura, Yusuke Sato, *Masahiro Takinoue, “Computational DNA droplets recognizing miRNA sequence inputs based on liquid-liquid phase separation”, Adv. Funct. Mater., 32, 2202322, (2022), DOI: 10.1002/adfm.202202322 |
| Organism | OTHERs |
| Cell (Tissue) | not applicable |
| Protocol | Sequence details are attached as a PDF file. |
| Data processing |
| [985] GMS_sequences_DNA-droplets.pdf
no description application/pdf 178.95 KB MD5: 11859d06fe103b3fd4692b55e3b39380 |
| [984] 1.jpg
no description image/jpeg 88.46 KB MD5: 8f25161134e9e47bef7771b4f27e2d23 |
