| Accession | GMS-82-4
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| Title | DNA condensates formed with DNA Tetrahedron structure and physical properties |
| Submit date | 2025-05-14 11:24:11 |
| Last update date | 2025-05-14 14:45:54 |
| Contact | Masahiro TAKINOUE masahiro.takinoue AT takinoue-lab.jp Institute of Science Tokyo |
| Sharing | |
| Total file size | 2.79 MB |
| Keywords | Nanoscale Synthetic DNA DNA droplet DNA nanotechnology DNA DNA condensate |
| Experiment type | In vitro experiments for the formation of artificial liquid-liquid phase separation droplets of DNAs |
| Summary | In this study, we report the formation of DNA condensates based on tetrahedron-shaped DNA nanostructures (Tetra-motifs). We designed an anisotropic Tetra-motif with two distinct pairs of sticky ends. Linkers corresponding to the stronger pair were introduced to form connections between Tetra-motifs. Unlike the flexible X-branched DNA nanostructures (X-motifs), the rigid and anisotropic structure of Tetra-motifs enabled their concatenation into extended, string-like structures. We found that these string-like structures of Tetra-motifs formed condensates even without cross-linking of multivalent motifs, relying solely on entanglement of these string-like structures. Mechanical and microfluidic experiments revealed that the resulting string-based condensates are highly deformable. Furthermore, we demonstrated the control of this string-based condensate by external stimuli, including UV irradiation and temperature changes, suggesting its potential as a stimuliresponsive material. |
| Citation(s) | Hong Xuan Chai, Kanta Kayanuma, Hiroaki Suzuki, *Masahiro Takinoue, “DNA condensates via entanglement of string-like structures based on anisotropic nano-tetrahedra”, (2025) |
| Organism | OTHERs |
| Cell (Tissue) | not applicable |
| Protocol | Sequence details are attached as a PDF file. |
| Data processing |
| [989] GMS_AFM_Tetra-motif.pdf
no description application/pdf 1.14 MB MD5: 81d7f2a9d95d7c310d4ea3ce2b288cc7 |
| [988] GMS_electrophoresis_Tetra-motif.pdf
no description application/pdf 811.05 KB MD5: 85a19c0c8a52c0b81b00b2c7fd1b9822 |
| [987] GMS_sequences_Tetra-motif.pdf
no description application/pdf 64.94 KB MD5: 00b9c818f567c2257112b6a2d5482dd8 |
| [986] Tetra-motifs.png
no description image/png 770.39 KB MD5: 874de0d332b93890d674e829e126dc52 |
