| Accession | GMS-82-5
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| Title | Sequence-dependent surface tension, viscosity, and inverse capillary velocity of DNA droplets |
| Submit date | 2025-05-14 14:19:19 |
| Last update date | 2025-05-14 14:46:09 |
| Contact | Masahiro TAKINOUE masahiro.takinoue AT takinoue-lab.jp Institute of Science Tokyo |
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| Total file size | 4.70 MB |
| Keywords | Nanoscale Synthetic DNA DNA droplet DNA nanotechnology DNA DNA condensate |
| Experiment type | In vitro experiments of measuring physical dynamic properties of artificial liquid-liquid phase separation droplets of DNAs |
| Summary | Liquid–liquid phase separation (LLPS) of biopolymer molecules generates liquid-like droplets. Physical properties such as viscosity and surface tension play important roles in the functions of these droplets. This study shows the changes in the physical properties of DNA droplets by sticky end (SE) design in DNA nanostructures. A Y-shaped DNA nanostructure (Y-motif) with three SEs as a model structure are used here. Seven different SE designs were used. The experiments were performed at the phase transition temperature where the Y-motifs self-assembled into droplets. We found that the DNA droplets assembled from the Y-motifs with longer SEs exhibited a longer coalescence period. In addition, the Y-motifs with the same length but different sequence SEs showed slight variations in the coalescence period. Our results suggest that the SE length greatly affected the surface tension at the phase transition temperature. |
| Citation(s) | *Yusuke Sato, *Masahiro Takinoue, “Sequence-dependent fusion dynamics and physical properties of DNA droplets”, Nanoscale Advances, 5, 1919-1925, (2023), DOI: 10.1039/D3NA00073G |
| Organism | OTHERs |
| Cell (Tissue) | not applicable |
| Protocol | Sequence details and physical properties are attached as a PDF file. |
| Data processing |
| [1021] GMS_sequences_physical-properties.pdf
no description application/pdf 3.71 MB MD5: bc7cab8ee106f121d7c053544ab8c5ba |
| [1018] Fig1.png
no description image/png 759.32 KB MD5: 290dd569fefcb0202c3f4c78322343fd |
| [1020] Fig2.png
no description image/png 106.93 KB MD5: b3649641e3b05e1b7a2e545786b40a81 |
| [1019] Fig3.png
no description image/png 120.19 KB MD5: ddf944122a79fa6e4f85831d947be22a |
