| Accession | GMS-82-7
URL copied to the clipboard |
| Title | Computational RNA droplets capable of AND operation upon specific microRNA inputs |
| Submit date | 2025-05-14 13:25:21 |
| Last update date | 2025-05-14 16:00:20 |
| Contact | Masahiro TAKINOUE masahiro.takinoue AT takinoue-lab.jp Institute of Science Tokyo |
| Sharing | |
| Total file size | 9.66 MB |
| Keywords | Synthetic RNA Computational Droplets X-motif RNA liquid condensate Nanoscale RNA DNA nanotechnology RNA nanotechnology |
| Experiment type | in vitro experiments to demonstrate logic-gate operation using syntheric RNA liquid condensates |
| Summary | Computational RNA droplets are composed of synthetic RNA nanostructures (motifs), self-asembled from multiple single-stranded RNAs (ssRNAs). The motifs form a network-like structure via kissing-loop (KL) interaction in the stem loop structure. To optimize the thermostability of the KL interaction, different KL candidate sequences are examined: KL_Ori, KL_Mut1, KL_Mut2 in the order of thermostability, in comparison with sticky-end type sequences. The designed motif comprises six ssRNA, four of which are engaged with KL, and the others have a toehold region of strand displacement reactions. They favor selectively bind with targeted microRNAs. Sequences are listed in the Supporing Material, uploaded below. |
| Citation(s) | H. Udono, M. Fan, Y. Saito, H. Ohno, S. M. Nomura, Y. Shimizu, H. Saito, M. Takinoue, "Programmable Computational RNA Droplets Assembled via Kissing-Loop Interaction", ACS Nano, 18, 24, 15477–15486. (DOI: https://doi.org/10.1021/acsnano.3c12161) |
| Organism | OTHERs |
| Cell (Tissue) | not applicable |
| Protocol | See Methods section in the cited paper and its Supporting Information, uploaded below. |
| Data processing |
| [1024] RNA_ACS_Nano_supporting.pdf
no description application/pdf 3.48 MB MD5: 4a48e8ea08a12a6ad2f5959e2c8cc67e |
| [1015] concept_Fig1.png
no description image/png 1.22 MB MD5: 6ec89f0497ad1d70b8e838200dbc4ccb |
| [1016] thermostability_Fig2.png
no description image/png 4.96 MB MD5: 08fb741a82f94c5b23547bcc14566569 |
