| Accession | GMS-64-2
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| Title | Generation of 3D Lacrimal Gland Organoids from Human Pluripotent Stem Cells |
| Submit date | 2022-01-31 13:43:30 |
| Last update date | 2025-03-31 16:41:30 |
| Contact | Sung-Joon PARK park AT hgc.jp The University of Tokyo |
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| Total file size | 1.34 MB |
| Keywords | Organoid Disease Sjogren's syndrome |
| Experiment type | Biological Sciences |
| Summary | Lacrimal glands are the main exocrine glands of the eyes. Situated within the orbit, behind the upper eyelid and towards the temporal side of each eye, they secrete lacrimal fluid as a major component of the tear-film. Here, we identify cells with characteristics of lacrimal gland primordia, which emerge in 2D eye-like organoids cultivated from human pluripotent stem cells. When isolated by cell sorting and grown under defined conditions, the cells form a 3D lacrimal gland-like tissue organoid with ducts and acini, enabled by budding and branching. Clonal colony analyses indicate that the organoids originate from multipotent ocular surface epithelial stem cells. The organoids exhibit notable similarities to native lacrimal glands on the basis of their morphology, immuno-labelling characteristics and gene expression patterns, and undergo functional maturation when transplanted above the eyes of recipient rats, developing lumina and producing tear-film proteins. |
| Citation(s) | https://pubmed.ncbi.nlm.nih.gov/35444274/ |
| Organism | Human |
| Cell (Tissue) | Larcrimal gland |
| Protocol | Life sciences techniques, Cell/tissue technologies [Flow cytometry]; Life sciences techniques, Cell/tissue technologies [Immunohistochemistry]; Life sciences techniques, Cell/tissue technologies [Stem cells]; Life sciences techniques, Gene expression analysis [RNA sequencing]; Life sciences techniques, Cell/tissue technologies [Antibodies]; Life sciences techniques, Cell/tissue technologies [Tissue engineering]; Life sciences techniques, Experimental organisms [Rats]; |
| Data processing | 3D organoid culturing Single-cell RNA sequencing |
| [169] D10_Cls0_1_10.png
Cell populations (Day 10) image/png 527.26 KB MD5: 294a8210745555d33fe8dfd0c7feb44a |
| [168] LacrimalGland.webm
LG-like organoid video/webm 815.73 KB MD5: ff88a186bd447a1af286d2468fc61688 |
