| Accession | GMS-65-2
URL copied to the clipboard |
| Title | Degradation of RAD21 in HCT-116 |
| Submit date | 2023-05-29 13:29:04 |
| Last update date | 2023-05-29 15:59:03 |
| Contact | GMSuite GENOMEMODALITY gmsuite AT hgc.jp The University of Tokyo |
| Sharing | |
| Total file size | 1.91 GB |
| Keywords | Cohesin ChIP-seq Micro-C EU-seq RAD21 |
| Experiment type | An auxin-inducible degron system destroys RAD21 in HCT-116 cell line. RAD21 is a core component of the cohesin complex.HCT-116 is a human colorectal carcinoma cell line. IAA untreated samples. |
| Summary | Before auxin Treatment (control) ChIP-seq AFF4, BRD4, CBP, CDK9, MAU2, MED1, NCBP1, NELFCD, RPB1, RAD21, S2P, SPT4, TAF1, TBP, P300, H3K27ac, H3K4me1 EU-seq in vivo 5-ethynyluridine (EU) labeling of nascent transcripts Micro-C PDB STAG with RAD21 https://gmsuite.hgc.jp/molstar/?pdb=6RRC AFF4 https://gmsuite.hgc.jp/molstar/?pdb=6R80 |
| Citation(s) |
| Organism | Human |
| Cell (Tissue) | HCT-116 |
| Protocol | |
| Data processing | ChIP-seq Bowtie2, MACS3 MM10 spike-in |
| [1] | EU-seq (control, Rep1) accepted_hits.bam.sorted.bam (1.87 GB) Human (hg38); RNA-Seq |
 BAM |
| [2] | AFF4 narrow peaks (control) AFF4_np1_peaks.narrowPeak.scaled.bigwig (992.08 KB) Human (hg38); ChIP-Seq |
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| [3] | BRD4 narrow peaks (control) BRD4_np1_peaks.narrowPeak.scaled.bigwig (3.30 MB) Human (hg38); ChIP-Seq |
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| [4] | CBP narrow peaks (control) CBP_np1_peaks.narrowPeak.scaled.bigwig (2.90 MB) Human (hg38); ChIP-Seq |
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| [5] | CDK9 narrow peaks (control) CDK9_np1_peaks.narrowPeak.scaled.bigwig (1.94 MB) Human (hg38); ChIP-Seq |
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| [6] | H3K27ac narrow peaks (control) H3K27ac_np1_peaks.narrowPeak.scaled.bigwig (5.42 MB) Human (hg38); ChIP-Seq |
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| [7] | H3K4me1 narrow peaks (control) H3K4me1_np1_peaks.narrowPeak.scaled.bigwig (7.56 MB) Human (hg38); ChIP-Seq |
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| [8] | MAU2 narrow peaks (control) MAU2_np1_peaks.narrowPeak.scaled.bigwig (3.23 MB) Human (hg38); ChIP-Seq |
BW |
| [9] | MED1 narrow peaks (control, rep1) MED1_np1_peaks.narrowPeak.scaled.bigwig (1.68 MB) Human (hg38); ChIP-Seq |
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| [10] | MED1 narrow peaks (control, rep2) MED1_np2_peaks.narrowPeak.scaled.bigwig (2.66 MB) Human (hg38); ChIP-Seq |
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| [11] | NCBP1 narrow peaks (control) NCBP1_np1_peaks.narrowPeak.scaled.bigwig (1.81 MB) Human (hg38); ChIP-Seq |
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| [12] | NELFCD narrow peaks (control) NELFCD_np1_peaks.narrowPeak.scaled.bigwig (2.35 MB) Human (hg38); ChIP-Seq |
BW |
| [13] | P300 narrow peaks (control) P300_np1_peaks.narrowPeak.scaled.bigwig (2.63 MB) Human (hg38); ChIP-Seq |
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| [14] | RAD21 narrow peaks (control) RAD21_np1_peaks.narrowPeak.scaled.bigwig (6.32 MB) Human (hg38); ChIP-Seq |
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| [15] | human cohesin subunit STAG1 in complex with RAD21 peptide 6RRC.png (237.36 KB) |
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