| Accession | GMS-65-2
URL copied to the clipboard |
| Title | Degradation of RAD21 in HCT-116 |
| Submit date | 2023-05-29 13:29:04 |
| Last update date | 2025-04-02 12:54:00 |
| Contact | GMSuite GENOMEMODALITY gmsuite AT hgc.jp The University of Tokyo |
| Sharing | |
| Total file size | 1.91 GB |
| Keywords | Cohesin Micro-C Disease CdLS Nuclear-scale AFF4 BRD4 MAU2 RAD21 SPT4 |
| Experiment type | An auxin-inducible degron system destroys RAD21 in HCT-116 cell line. RAD21 is a core component of the cohesin complex.HCT-116 is a human colorectal carcinoma cell line. IAA untreated samples. |
| Summary | Before auxin Treatment (control) ChIP-seq AFF4, BRD4, CBP, CDK9, MAU2, MED1, NCBP1, NELFCD, RPB1, RAD21, S2P, SPT4, TAF1, TBP, P300, H3K27ac, H3K4me1 EU-seq in vivo 5-ethynyluridine (EU) labeling of nascent transcripts Micro-C PDB STAG with RAD21 https://gmsuite.hgc.jp/molstar/?pdb=6RRC AFF4 https://gmsuite.hgc.jp/molstar/?pdb=6R80 |
| Citation(s) |
| Organism | Human |
| Cell (Tissue) | HCT116 |
| Protocol | |
| Data processing | ChIP-seq Bowtie2, MACS3 MM10 spike-in |
| [389] | EU-seq (control, Rep1) Browse Browse (All) Human (hg38); RNA-Seq |
 BAM accepted_hits.bam.sorted.bam (1.87 GB) |
| [347] | AFF4 narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW AFF4_np1_peaks.narrowPeak.scaled.bigwig (992.08 KB) |
| [350] | BRD4 narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW BRD4_np1_peaks.narrowPeak.scaled.bigwig (3.30 MB) |
| [353] | CBP narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW CBP_np1_peaks.narrowPeak.scaled.bigwig (2.90 MB) |
| [368] | CDK9 narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW CDK9_np1_peaks.narrowPeak.scaled.bigwig (1.94 MB) |
| [385] | H3K27ac narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW H3K27ac_np1_peaks.narrowPeak.scaled.bigwig (5.42 MB) |
| [386] | H3K4me1 narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW H3K4me1_np1_peaks.narrowPeak.scaled.bigwig (7.56 MB) |
| [371] | MAU2 narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW MAU2_np1_peaks.narrowPeak.scaled.bigwig (3.23 MB) |
| [372] | MED1 narrow peaks (control, rep1) Browse Human (hg38); ChIP-Seq |
BW MED1_np1_peaks.narrowPeak.scaled.bigwig (1.68 MB) |
| [369] | MED1 narrow peaks (control, rep2) Browse Human (hg38); ChIP-Seq |
BW MED1_np2_peaks.narrowPeak.scaled.bigwig (2.66 MB) |
| [370] | NCBP1 narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW NCBP1_np1_peaks.narrowPeak.scaled.bigwig (1.81 MB) |
| [367] | NELFCD narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW NELFCD_np1_peaks.narrowPeak.scaled.bigwig (2.35 MB) |
| [373] | P300 narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW P300_np1_peaks.narrowPeak.scaled.bigwig (2.63 MB) |
| [374] | RAD21 narrow peaks (control) Browse Human (hg38); ChIP-Seq |
BW RAD21_np1_peaks.narrowPeak.scaled.bigwig (6.32 MB) |
| [346] | human cohesin subunit STAG1 in complex with RAD21 peptide 6RRC.png (237.36 KB) |
|
| [389] accepted_hits.bam.sorted.bam
EU-seq (control, Rep1) application/octet-stream 1.87 GB MD5: a45bdbdfa7b77e3d1dc4efed3e84db52 |
| [347] AFF4_np1_peaks.narrowPeak.scaled.bigwig
AFF4 narrow peaks (control) application/octet-stream 992.08 KB MD5: c8faf1c4a7116b88b416596b35b3d896 |
| [350] BRD4_np1_peaks.narrowPeak.scaled.bigwig
BRD4 narrow peaks (control) application/octet-stream 3.30 MB MD5: f4e4ce88a672ad7585c58e9334a9906a |
| [353] CBP_np1_peaks.narrowPeak.scaled.bigwig
CBP narrow peaks (control) application/octet-stream 2.90 MB MD5: 3ca2a82f1c587a1194f16052bfb74013 |
| [368] CDK9_np1_peaks.narrowPeak.scaled.bigwig
CDK9 narrow peaks (control) application/octet-stream 1.94 MB MD5: 31d02fd07ba6e1a1f4bb6a768c29cd76 |
| [385] H3K27ac_np1_peaks.narrowPeak.scaled.bigwig
H3K27ac narrow peaks (control) application/octet-stream 5.42 MB MD5: 923dcd87bea5ed6047b12ca7f2e4a96c |
| [386] H3K4me1_np1_peaks.narrowPeak.scaled.bigwig
H3K4me1 narrow peaks (control) application/octet-stream 7.56 MB MD5: 94d416f0e5e2a69c2a0f285f2e9070d1 |
| [371] MAU2_np1_peaks.narrowPeak.scaled.bigwig
MAU2 narrow peaks (control) application/octet-stream 3.23 MB MD5: a07676db23f1c8ae89fdc014f09068fd |
| [372] MED1_np1_peaks.narrowPeak.scaled.bigwig
MED1 narrow peaks (control, rep1) application/octet-stream 1.68 MB MD5: 3f991ecc4f088c03afcb98fd9539dbe7 |
| [369] MED1_np2_peaks.narrowPeak.scaled.bigwig
MED1 narrow peaks (control, rep2) application/octet-stream 2.66 MB MD5: b3e1b8ded31c779934eb7350b9bd0fcb |
| [370] NCBP1_np1_peaks.narrowPeak.scaled.bigwig
NCBP1 narrow peaks (control) application/octet-stream 1.81 MB MD5: c86b20d429fab3492db33d58a653cc46 |
| [367] NELFCD_np1_peaks.narrowPeak.scaled.bigwig
NELFCD narrow peaks (control) application/octet-stream 2.35 MB MD5: 277dbd96f53a48c7f3a20ff6eb9d8bad |
| [373] P300_np1_peaks.narrowPeak.scaled.bigwig
P300 narrow peaks (control) application/octet-stream 2.63 MB MD5: 3f62901df842f3d6d289d3bb19131792 |
| [374] RAD21_np1_peaks.narrowPeak.scaled.bigwig
RAD21 narrow peaks (control) application/octet-stream 6.32 MB MD5: 2119055789f389a6a43e7cb3e626fd0d |
| [346] 6RRC.png
human cohesin subunit STAG1 in complex with RAD21 peptide image/png 237.36 KB MD5: bc9978608a3a54afc7d5fd7e16384abf |
