| Accession | GMS-82-6
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| Title | Temporally controlled division of DNA droplets coupled with enzymatic reaction |
| Submit date | 2025-05-14 16:33:09 |
| Last update date | 2025-05-14 17:01:36 |
| Contact | Masahiro TAKINOUE masahiro.takinoue AT takinoue-lab.jp Institute of Science Tokyo |
| Sharing | |
| Total file size | 22.19 MB |
| Keywords | Nanoscale Synthetic DNA DNA droplet Division Y-motif DNA linker |
| Experiment type | In vitro experiments for the division of DNA dropletscoupled with enzymatic reactions. |
| Summary | DNA droplets are formed by the self-assembly of orthogonal Y-shaped DNA nanostructures (Y-motifs) and DNA linkers that crosslink Y-motifs. The cleavage of the DNA linkers by hybridizing with complementary sequences of miRNAs results in the division of DNA droplets. The timing control of DNA droplet division was demonstrated by controlling the release rate of complementary sequences of miRNAs from the miRNA/DNA hybrid by ribonuclease H-based enzymatic reactions. Additionally, controlling the release order of multiple complementary sequences of miRNAs corresponding to distinct DNA linkers induced order control of DNA linker cleavage, resulting in the multistep droplet division, i.e., pathway-controlled division in a reaction landscape. Furthermore, the fluidity of single Y-motif-based DNA droplets was lower than that of DNA droplets containing multiple Y-motifs and DNA linkers due to high multi-valency of the linker having 6 branches. |
| Citation(s) | Tomoya Maruyama, Jing Gong, Masahiro Takinoue, “Temporally controlled multistep division of DNA droplets for dynamic artificial cells”, Nature communications., 7397, 2024, 15, DOI: https://doi.org/10.1038/s41467-024-51299-5 |
| Organism | OTHERs |
| Cell (Tissue) | not applicable |
| Protocol | All experiments are carried out in a buffer containing 350 mM NaCl, 20 mM Tris-HCl (pH 8.0), and 1.5 mM MgCl2 at 60 or 63 °C. For more information, see the Methods section and Supplementary Information in the published article. |
| Data processing |
| [1030] GM_sequence.pdf
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| [1029] GM_fig1.png
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| [1025] GM_Fig5.png
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| [1036] Supplemental_Movie_S1.mp4
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| [1037] Supplemental_Movie_S9.mp4
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