| Accession | GMS-82-3
URL copied to the clipboard |
| Title | Photo-responsive DNA condensates |
| Submit date | 2025-05-14 12:17:15 |
| Last update date | 2025-05-14 14:45:32 |
| Contact | Masahiro TAKINOUE masahiro.takinoue AT takinoue-lab.jp Institute of Science Tokyo |
| Sharing | |
| Total file size | 41.01 MB |
| Keywords | Synthetic DNA Nanoscale DNA condensate Y-motif DNA linker Photo-control DNA fluid DNA DNA nanotechnology DNA droplet |
| Experiment type | In vitro experiments for the formation of photo-responsive DNA condensates and their mechanical actions |
| Summary | Photo-reactive DNA condensatres are composed of Y-shaped DNA nanostructures (Y-motif), self-assembled from three azobenzene-modified ssDNAs (listed below). The basic sequences are referenced from Y. Sato et al., Sci. Adv. (2020) (DOI: 10.1126/sciadv.aba3471). The azo DNA condensates is capable of reversible phase-state changes upon irradiation of UV or visibile light (Vis). UV leads to an increase in the fluidity (gel-to-liquid or gel-to-dispersed state changes); Vis leads to a decrease in the fluidity (disperse-to-liquid). Thee types of azo sequnces are highlighted: Y_1x7; Y_3x5; Y_2x1x5, in the order of thermostability, wher 'x' refers to the azo insertion site in the SE. When coupled with non-photoresponsive DNA using linker DNA, the resulting condensates show self-collapsing behavior (1x7) and burst-like flow and disassembly–reassembly cycle flow (3x5, 2x1x5) |
| Citation(s) | H. Udono, S. M. Nomura, M. Takinoue, Nature Communications (accepted, 2025): DOI: 10.26434/chemrxiv-2022-0n2fm |
| Organism | OTHERs |
| Cell (Tissue) | not applicable |
| Protocol | The buffer condition is the same as that of Y. Sato et al. (2020). See the Methods section and Supplementary Information in the published article. |
| Data processing |
| [1006] list_of_sequences.pdf
no description application/pdf 199.83 KB MD5: 1e142047d46a1055c60f8f56ae9dbae2 |
| [1007] native_PAGE.pdf
no description application/pdf 1.76 MB MD5: 46b3cc72464e4a733e126d74e483b44a |
| [1008] MovieS1_UV_induced_gel_to_liquid_phase_transition.mp4
no description video/mp4 1.62 MB MD5: 6831f7a6f8563871847c12ebffaeeee7 |
| [1009] MovieS2_Vis_induced_dispersed_to_gel_phase_transition.mp4
no description video/mp4 2.70 MB MD5: 6a3bcb2aadd4a704d9574d0a59559d4d |
| [1010] MovieS3_burst_light.mp4
no description video/mp4 4.44 MB MD5: 9823df6ed5f652de5d586c50cb7551b9 |
| [1012] MovieS4_collapse.mp4
no description video/mp4 25.50 MB MD5: 046437a1d4a15862338633bd7e23fb42 |
| [1011] MovieS5_reversing_lighter.mp4
no description video/mp4 4.79 MB MD5: f8645c03aedeaa6b1a348199339ebc10 |
